Affinity

Proteomics Services

Affinity

Affinity pull-downs are one of the most common types of experiments performed in the laboratory. Combining such experiments with MS can be challenging but it is a very powerful approach for studying protein interactions.

Understanding protein-protein interactions in biological pathways is an essential part of the overall comprehension of all biological processes. Mass-spectrometry proteomics is a powerful tool for analysis of interactomes. Unlike WB analysis it can identify all proteins purified in an unbiased way. This can present disadvantages as contaminants can become a serious challenge.

Although some analyses can be performed on unlabelled samples, SILAC labelling can greatly improve data quality and help identify contaminants in Affinity Pull-Down experiments.DC Biosciences offer a range of Pull-Down experiments, from variants of Immuno-Precipitations to so-called “proximity proteomics”. Our experts will accompany you from experiment design to completion, helping you avoid the many pitfalls of Pull-Down MS analysis. We will ensure you are able to extract the greatest possible value from your data.

Proteomics Services from DC Biosciences

  • Dedicated and enthusiastic proteomics experts
  • State of the art proteomics technologies
  • Expert guidance from experiment design to results interpretation
  • Continual interaction at all stages of the process
  • SILAC CO-IP

    Leverage the power of SILAC to discriminate real hits from
    contaminants in your co-IP experiments.

  • GFP SILAC CO-IP

    Don’t have a good antibody to your protein? Express a GFP-tagged fusion, then perform a SILAC co-IP.

  • BIO-ID

    Proximity proteomics: complementary to co-IPs, detect proteins within a radius of your protein of interest.

  • IDS FROM IP

    Perform an unlabelled IP then compare with a list of common known contaminants to identify true hits.

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