TMT goes 11-plex!

Thermo Fisher releases TMT 11-plex. Thermo Fisher recently released an additional channel for TMT, which means you can now combine up to 11 different samples as one for better sample comparability and more complete data! It is now possible to use “isotopologue” encoding to split the 131 (last) channel into 131-N and 131-C to get […]

ADAPTED Project Press Release

New project to explore overlooked area of Alzheimer’s Disease research Alzheimer’s disease (AD) affects over 35 million people world-wide at present, these numbers are expected to grow substantially over the next few decades and there are currently no medicines which slow the progress of the disease. To boost the development of new medicines for AD, […]

Dr. Francesco Rao
About LC-MS/MS
Database Searching

Ideally, each MS2 spectrum would contain enough information to go back unambiguously to the sequence of its parent precursor. Since this is actually rarely the case, a strategy called Database Searching is usually used instead. MS2 fragmentation spectra are used to identify the sequence behind their MS1 parent peak. However, MS2 spectra rarely allow unambiguous […]

Dr. Armel Nicolas
About LC-MS/MS
Data Acquisition

Data Dependent Acquisition (DDA) is the standard method for running LC-MS/MS experiments. Here, we present a quick overview of the DDA method, as well as of the less common Data Independent methods (DIA). In LC-MS/MS, purified peptides are separated on an online chromatography gradient (usually low pH Reverse Phase) over 1 to 4h and progressively […]

Dr. Armel Nicolas
Point of View