Affinity pull-downs are one of the most common types of experiments performed in the laboratory. Combining such experiments with MS can be challenging but it is a very powerful approach for studying protein interactions.
Understanding protein-protein interactions in biological pathways is an essential part of the overall comprehension of all biological processes. Mass-spectrometry proteomics is a powerful tool for analysis of interactomes. Unlike WB analysis it can identify all proteins purified in an unbiased way. This can present disadvantages as contaminants can become a serious challenge.
Although some analyses can be performed on unlabelled samples, SILAC labelling can greatly improve data quality and help identify contaminants in Affinity Pull-Down experiments. DC Biosciences offer a range of Pull-Down experiments, from variants of Immuno-Precipitations to so-called “proximity proteomics”. Our experts will accompany you from experiment design to completion, helping you avoid the many pitfalls of Pull-Down MS analysis. We will ensure you are able to extract the greatest possible value from your data.
- Dedicated and enthusiastic proteomics experts
- State of the art proteomics technologies
- Expert guidance from experiment design to results interpretation
- Continual interaction at all stages of the process